Detection of cannabinoids in hair after cosmetic application of hemp oil Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, Hemp seed oilprotects your hair from damage and dryness and reduces scalp inflammation. Here are the potential benefits of hemp seed oil for your hair. Curious about hemp oil for hair health? Here’s what you need to know about how to use it and what the research says about hemp oil versus hemp seed oil.
Detection of cannabinoids in hair after cosmetic application of hemp oil
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
All data generated or analyzed during this study are included in this published article.
The detection of cannabis constituents and metabolites in hair is an established procedure to provide evidence of exposure to cannabis. We present the first known evidence to suggest that applying hemp oil to hair, as cosmetic treatment, may result in the incorporation of Δ 9 -tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD) and in one instance, the metabolite 11-hydroxy-Δ 9 -tetrahydrocannabinol (THC-OH). 10 volunteers treated their head hair daily with commercially available hemp oil for a period of 6 weeks. Head hair samples were collected before and after the application period. Hair samples were washed with methanol and subjected to clean up via liquid/liquid and solid phase extraction procedures, and then GC-MS/MS for the analysis of THC, CBN, CBD, THC-OH and THC-COOH. Application of hemp oil to hair resulted in the incorporation of one or more cannabis constituents in 89% of volunteers, and 33% of the group tested positive for the three major constituents, THC, CBN and CBD. One volunteer showed low levels of the metabolite THC-OH. We suggest that cosmetic use of hemp oil should be recorded when sampling head hair for analysis, and that the interpretative value of cannabinoid hair measurements from people reporting application of hemp oil is treated with caution in both criminology and public health.
Cannabis Sativa is a plant species of Cannabis. In addition to its recreational use as a drug of abuse, the plant has widespread alternative uses including the production of food, cosmetics (hemp), textiles and medicinal applications 1 . When toxicology laboratories are required to investigate past exposure to cannabis, analysis of hair can provide powerful evidence. The compounds usually targeted for hair analysis to identify cannabis exposure are: Δ(9)-tetrahydrocannabinol (THC), the main active compound of cannabis, the metabolite [11-nor-Δ(9)-tetrahydrocannabinol-9-carboxylic acid (THC-COOH)] and two cannabinoids (cannabinol (CBN) and cannabidiol (CBD)) 2 . Typically passage of these cannabinoids into the hair includes passive diffusion from blood, diffusion from sweat/sebum or external contamination. One of the key questions to be addressed when interpreting the results of cannabinoid hair analysis is that of proof of consumption. Are the results sufficiently clear to suggest cannabis was consumed, or could the results actually be the result of passive exposure to cannabis smoke, or other mechanisms? Passive exposure is defined by an individual being in an environment that is exposed to drugs, an important public health problem. Cannabis smoke can be inhaled or absorbed into the hair by persons other than the intended smoker/user 3 . Researchers have evaluated second-hand cannabis smoke exposure and the corresponding levels of cannabinoids in biological samples 3 , 4 . Herrmann et al. discovered that in unventilated, confined conditions cannabinoid detection was above threshold and higher concentrations of THC and THC-COOH were found predominantly in the blood, urine and hair 4 . THC and THC-COOH have lower incorporation rates in hair in comparison to other bodily matrices. The low presence of THC may be explained by its weak affinity to melanin while the acidic nature of hair may be the reason for the absence of THC-COOH 5 . Along with the levels of cannabis constituents detected in passive exposure, analysis has been conducted to understand what physiological impact exposure has 3 . Past research has shown evidence of increased heart rate and minor impairments in coordination and memory 4 , 6 , 7 . Identification of THC/CBN/CBD in hair suggests exposure to cannabis, which could be due to low level or infrequent use of cannabis or historic or passive exposure. However, some argue that the presence of cannabinoids in hair, especially THC is indicative of repeated or chronic exposure 5 , 8 . The distinction between external contamination and consumption can be difficult for cannabinoid hair analysis 9 , and the implication of a positive test result can have significant consequences for the individual involved. THC-COOH is only formed inside the body, and the presence of this gives unequivocal proof of consumption when detected in hair samples. The metabolite has never been discovered in cannabis smoke ruling out environmental contamination 10 . With hair analysis, THC-COOH is detectable at very low concentrations. The drawbacks for detection from this biological matrix are the requirement for expensive instrumentation and sample preparation can be a more time-consuming process when compared to urine 11 . Routine laboratory screening of hair for cannabis varies and includes the detection of cannabinoids and/or THC-COOH 8 . Hemp is a variety of Cannabis Sativa and is closely related to Cannabis with the difference being in the percentage of THC 12 . Hemp is grown for industrial use and found in food, lotions, medicines, clothing and construction materials. Hemp oil is extracted by pressing the seeds from the female hemp plant 13 . The legalisation of hemp has caused controversy. This is because research has shown that the use or consumption of hemp products could have the potential to impact on drug testing for cannabis 14 .
Hemp oil products are advertised in health shops for their good source of omega fatty acids 15 . Bosy et al. 16 assessed whether oral consumption of hemp oil would negatively affect existing drug screening protocols. Various oils were screened (THC content of bottled oils was 36.0, 117.5, 36.4, 45.7, 21.0, 11.5 mg/g) and administered to volunteers and their urine measured for metabolite levels. GC-MS analysis determined the amount of THC-COOH in each participant’s urine to be below the confirmation cut-off within a 48 hour cessation period. Similarly to hemp oil, hemp foods are classified as ‘natural foods’ and are commercially available. Leson et al. showed that daily consumption of hemp food can lead to the presence of THC and THC-COOH in urine, but these compounds were below the confirmation thresholds 17 . These authors 16 , 17 suggest that hemp food and oil products do contain cannabinoids but in very low concentrations, and that ingestion of such products should not be deemed as a concern in drug testing. The Cannabis plant has been used in the production of cosmetics through the use of hemp oil and cannabis extracts 18 . An evaluation of Cannabio® shampoo revealed levels of THC, CBD and CBN, three constituents that indicate cannabis exposure 19 . However, normal hygiene practice using the cosmetic produced no positive results in hair. Extreme use could generate positive results for CBN and CBD but not the primary constituent, THC.
Hemp oil is marketed as an effective cosmetic treatment for hair, with claims that direct application of the oil to hair has moisturizing benefits, can aid hair growth, may protect the hair and aid in damage repair, and the oil may add shine to the hair. These claims are unsubstantiated but there is a substantial number of online retailers selling various hemp oil based products intended for direct application to head hair. The composition of these products range from pure hemp oil, to hemp oil included at a relatively low concentration into shampoos and other hair treatments.
In this paper we investigate direct hemp oil application to head hair and the implications on resulting cannabinoid measurements.
Cannabinoid concentrations pre and post hemp oil application
Head hair samples were collected from volunteers as described in Methods, and analysed before and after the six week period of hemp oil administration. Results are displayed in Table 1 .
Hemp Seed Oil For Hair – 4 Benefits And How To Use It
Improving hair health, reducing breakage, and more ways this oil keeps your locks happy!
Tiffany Young is the CEO and founder of ThinHairThick. She is also a recognized expert in the hair and beauty industry with celebrity clientele. Her own personal struggles with hair loss created an opportunity for her to deep-dive into lear. more
Arshiya Syeda is an editor at StyleCraze. Prior to that, she was a content writer and combined her writing and research skills to write over 200 high-performing articles on hairstyles, hair care, and . more
Hemp seed oil can do great things for your hair. It is extracted from hemp seeds and has a clear green color. Cannabis sativa (hemp) plant has a relaxing effect. Using hemp seed oil for hair has become a popular practice in recent years. Whether it is dry hair, rough hair, damaged hair, or scalp inflammation – hemp seed oil can help nourish and soothe your strands and the scalp. Hemp vitamins, proteins, and essential fatty acids make your hair healthy and lustrous. Read on to learn all the benefits of hemp seed oil for hair and how to use it. Scroll down!
In This Article
Benefits Of Using Hemp Seed Oil For Hair
There is much anecdotal evidence for the benefits of hemp seed oil. It is said to possess antibacterial properties, treat scalp infections, stimulate hair growth, moisturize the scalp, and prevent breakage. Read on to know more about these benefits in detail.
Why Hemp Oil Can Help Strengthen Your Hair & Scalp + How To Use It
Jennifer Chesak is a freelance medical journalist with bylines in several national publications, including Washington Post, Healthline, Prevention, Greatist, Runner’s World, and more. She earned her Master of Science in journalism from Northwestern’s Medill School.
Most people know hemp for its calming, relaxing benefits—but that’s not all it’s good for. Whether taken as a supplement or used topically, both hemp oil extract (which contains beneficial cannabinoids) and hemp seed oil (which is cold-pressed from hemp seeds and does not contain cannabinoids) can deliver benefits to the hair and scalp. Here’s how hemp products can help you achieve healthier hair, according to research.
What does the science say about using hemp extract for healthier hair?
Hemp oil extract is rich in polyunsaturated fatty acids such as omega-3 and omega-6 fatty acids. Your body can’t make these nutrients naturally, but it needs them either through diet or supplementation. When it comes to our manes, these omega-3s and omega-6s may help manage hair loss, help maintain the thickness of the hair shaft (the part that you can see above the scalp), and support hair density, according to one study that tracked the hair health of over 100 female participants over a six-month period.*
Hemp oil extract is also high in antioxidants that protect our cells from damage by combating the effects of free radicals and oxidative stress.* Plus, as long as the product is full-spectrum, hemp oil has an abundance of terpenes and flavonoids that can help manage inflammation as well.* When it comes to your tresses, hemp oil also contains vitamins and minerals that have the potential to protect against damage and hair loss, though more research needs to be done into how exactly they work in the body.*
Hemp oil extract can be taken as a daily supplement to nourish your hair and scalp—and it also comes with a host of other ingredients that can be beneficial to overall well-being.* CBD, for example, can help keep the body in homeostasis.* And when CBD works in concert with the hundreds of other compounds in the cannabis plant, you benefit from what’s called the entourage effect, a synergy of ingredients working together to keep you and your body on an even keel.*
What does the science say about using hemp seed oil for healthier hair? (Plus, how to use it for your hair type.)
Like other oils, hemp seed oil may have protective qualities when applied directly to the hair and scalp. We have long known that oil can prevent too much water or other substances from invading the hair shaft. The more porous hair is from chemical damage or over-styling, the more vulnerable it is to water’s wear. Water can be an enemy to locks because wet tresses swell and become more susceptible to breakage. Oil also helps manage hair harm by lubricating the shaft and reducing combing force on soaked strands.
With hemp seed oil, the protection starts with the essential fatty acids omega-3 and omega-6. Fatty acids have a hydrophilic end that aids in moisture retention, and a hydrophobic end that boosts shine, “making hemp seed oil a perfect product for coarse, curly hair,” says Austin-based stylist Sarah Lund.
Additionally, vitamin E also plays a role in fending off the elements and battling damage. “This will create greater elasticity in the hair while adding shine,” Lund explains.
You can tailor your use of hemp seed oil to your hair type. For dry, coarse, or curly hair, use the product as a daily leave-in moisturizer. “A small amount distributed through hair midshaft [the middle of your strands] to ends will help to strengthen and moisturize hair that is older and more vulnerable to styling damage,” says Leigh Hardges, a Chicago-based stylist. “Those with straight or fine hair that steer clear of daily oils could benefit from hemp seed oil as a hot oil treatment.” For a dry or oily scalp, Hardges recommends applying the hemp seed oil directly to the scalp overnight and then shampooing in the morning. “The amino acids in hemp seed oil can deliver properties to help balance the scalp and regulate the production of sebum, the body’s natural oil,” she explained.
The bottom line.
If you’ve been looking for an all-natural way to up your hair and scalp health, hemp oil extract and hemp seed oil could be great options.* Hemp oil extract can be taken as a daily supplement for added nutrition that helps manage hair loss and promotes thickness and density.* And hemp seed oil can be applied directly to your hair and scalp to moisturize and combat breakage. Or take a two-pronged approach. Enhance your hair from both the inside and out by using both products to produce happy, healthy tresses.